MSRE-PCR for analysis of gene-specific DNA methylation
نویسندگان
چکیده
منابع مشابه
MSRE-PCR for analysis of gene-specific DNA methylation
Abnormal DNA methylation is observed in certain promoters of neoplastic cells, although the likelihood of methylation for each individual promoter varies. Simultaneous analysis of many promoters in the same sample can allow use of statistical methods for identification of neoplasia. Here we describe an assay for such analysis, based on digestion of genomic DNA with methylation-sensitive restric...
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Gastric Cancer (GC) is the second most common cancer in the world and a leading cause of cancer-related mortality. Methylation of promoter CpG islands (CGIs) belonging to tumor suppressor genes causes transcriptional silencing of their corresponding genes leading to carcinogenesis and other disorders. Adenomatous Polyposis Coli (APC) a tumor suppressor gene is inactivated by methylation of prom...
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Methylation-specific PCR (MSP) of the mouse p53 gene has not yet been reported. We searched the CpG islands, sequenced the bisulfited DNA, and designed PCR primers for methylation and unmethylation sites. DNA from a young mouse produced a strong PCR product with the unmethylated primer and a weaker band with the methylated primer. DNA from an old mouse produced bands of similar intensities with...
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متن کاملMethylation-specific PCR.
Methylation of the DNA is an important epigenetic (i.e., not associated with alteration in the primary structure of the DNA) phenomenon, which plays important roles in regulation of gene expression, maintenance of genome integrity, and genomic imprinting. Although not only other nucleotides, but also proteins and lipids can be methylated, in the context of the present discussion, “methylation” ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2005
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gni092